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. 2021 Mar 15;17(5):1178–1190. doi: 10.7150/ijbs.57783

Figure 7.

Figure 7

CDK1 is the target of miR-31-5p and indirectly regulated by circMETTL3. A. The Venn-diagram indicates the number of genes that overlapped in three publicly available bioinformatics algorithms (Targetscan, mirDIP, miTarbase) and top 300 upregulated genes in breast cancer downloaded from GEPIA (GEPIA). B-C: The mRNA and protein expression levels of CDK1 in ZR-75-1 (B) and SUM1315(C) cell lines transfected with miR-31-5p mimic (miR-31-5p) or miR-31-5p inhibitor (inh-31-5p). D. The relative luciferase activities were detected in 293T cells after transfected with CDK1 3'UTR-WT or CDK1 3'UTR-Mut and miR-31-5p mimics or miR-NC, respectively. E. Expression of CDK1 in breast cancer cells after knockdown of circMETTL3. F-G: qRT-PCR and western blot were excused to detect CDK1 expression in ZR-75-1 (F) and SUM1315 (G) cell lines transfected with negative control (si-NC), si-circMETTL3 (si-circ) or circMETTL3+miR-31-5p inhibitor(si-circ+inh). Data were shown as mean ± SD, *p<0.01, **p <0.01, ***p<0.001.