Figure 5.

MnSOD-K68-Ac exhibits higher peroxidase activity when incorporated with iron. (a.b) The expression of purified MnSOD-WT and MnSOD-K68-Ac protein was verified by Coomassie blue (a) staining (as arrow pointed) and immunoblotting (b) for MnSOD and MnSOD-K68-Ac. (c) 10 µg purified protein of MnSOD-WT or MnSOD-K68-Ac was loaded with 200 µM Mn²⁺, or 200 µM Fe²⁺ was measured for enzymatic peroxidase activity. (d) pMEF cells were transfected with FLAG-MnSOD^WT, FLAG-MnSOD^K68R, or FLAG-MnSOD^K68Q, and treated with 200 µM Fe²⁺ with or without 25µM deferoxamine (DFO) cell lysates were incubated with anti-FLAG beads. Pulled-down MnSOD^WT, MnSOD^K68R and MnSOD^K68Q were measured for enzymatic peroxidase activity. Representative images are shown. All experiments were done in triplicates. Error bar represents ±1 SEM. *p<0.05, ***p<0.001.