Figure 2.

PAK5 directly binds to AIF. (a,b) Endogenous AIF interacts with PAK5. BT474 cells/T47D lysates were immunoprecipitated with the anti-PAK5, anti-AIF or IgG. Precipitates were analyzed by western blot. (c) Exogenous PAK5 interacts with AIF. Total lysates were subjected to immunoprecipitation and western blot. (d) PAK5 directly binds to GST-AIF in vitro. Black stars indicate GST and GST-fusion proteins. (e) Co-localization of PAK5 and AIF. Nucleus was stained with DAPI (4', 6 diamidino-2-phenylindole). Yellow indicates co-localization. Original magnification, ×600. The Pearson's correlation and overlap co-efficient were shown in bar graph format (30 cells) from three independent experiments were analyzed (error bars, SEM).