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TLR4 is involved in miR-4485-3p-mediated osteogenic differentiation. (A) The luciferase assay of miR-4485-3p and TLR4. (B-E) The level of TLR-4 was measured by qRT-PCR and western blot analysis. (F-G) The level of osteogenic-related mRNAs in different groups was measured by western blot analysis. (H-I) The alizarin red staining and ALP staining results of different groups. Data are the mean ± SD 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001.
