Figure 7.

Local delivery of miR-4485-3p antagonist rescues the delayed fracture healing phenotype in mice. (A) Fractures in the siRNA-TLR4+AntagomiR-4485-3p-treated mice had reduced cartilage and increased bone compared with siRNA-TLR4-treated control fractures. (B) Histomorphometry showed that fractures in the AntagomiR-4485-3p-treated mice were composed of a markedly high proportion of bone, and low proportion of cartilage. In contrast, fractures in the siRNA-TLR4-treated mice were composed of low bone density and high cartilage density. (C-D) siRNA-TLR4-treated fractures in mice had increased callus area and decreased bone area compared with AntagomiR-4485-3p-treated mice. *p<0.05, **p<0.01, ***p<0.001.