Fig. 7.

Effect of CoQ₁₀ ND on oxygen consumption rates (OCR) of C2C12 myotubes. Differentiated C2C12 myotubes were incubated with DMEM supplemented with either 20 μM CoQ₁₀ ND (open bars) or an equivalent amount of empty ND (filled bars) for 72 h. Following incubation, cells were subjected to a mitochondrial stress test assay as described in the materials and methods. Upon completion of the mitochondrial stress test, OCR were determined for each well and normalized to nucleus count (as visualized following DAPI stain). The graph depicts (1) basal respiration rates and (2) maximal respiration rates for C2C12 cells incubated with control empty ND (filled bars) or with CoQ₁₀ ND (20 μM CoQ₁₀). Values reported are the mean ± standard error (n = 3) * p < 0.05 vs empty PtdCho ND. DMEM, Dulbecco’s minimal essential medium