Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 8;218(6):e20202359. doi: 10.1084/jem.20202359

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Vijay et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Plasmablasts can expand independently of antigen–BCR interactions. (A–D) WT mice were infected with 10⁶ Py pRBCs. On days 4, 7, and 10 p.i., parasitemia, plasmablasts (CD138^hiIgD^neg), activated B cells (CD138^loIgD^neg), and naive splenic B cell populations (CD138^loIgD^hi) were quantified (n = 19). Using other WT mice (n = 4), the three B cell populations were sort-purified on day 10 p.i. and RNA sequenced for transcriptomic analysis. (A) Representative gating strategy showing plasmablasts, activated B (Act B) cells, and naive splenic B cells in either naive (left panel) or Py-infected mice (middle panel) on day 10 p.i. (B) Correlation graph showing frequency of plasmablasts (x axis) compared with frequency of infected RBCs (% parasitemia, y axis). (C and D) Canonical pathways and upstream regulators of each phenotype were identified using Ingenuity Pathway Analysis software. (C) Pathways down-regulated (negative activation z score) in CD138^hi plasmablasts compared with activated B cells. (D) Left panel: Upstream regulators defining activated B cells (red) in comparison to CD138^hi plasmablasts (blue). Right panel: Upstream regulators defining naive B cells (green) in comparison to CD138^hi plasmablasts (blue). (E) WT and MD4 mice were infected with 10⁶ Py pRBCs. Representative plots, proportions, and total numbers of CD138^hiIgD^neg plasmablasts on day 10 p.i. n = 5 or 6 mice/group, mean ± SEM, representative of two biologically independent experiments, analyzed by Mann-Whitney. (F) Parasitemia on day 10 p.i. (G) Anti–parasite lysate–specific IgM and IgG (top row) and anti-HEL–specific IgM and IgG (bottom row) antibody responses in sera from day 10 p.i. Data are mean ± SEM, representative of six independent experiments, analyzed using two-way ANOVA. n = 6 mice/group, mean ± SEM, representative of two biologically independent experiments, analyzed by Mann-Whitney. (H) Treemap diagrams of VH gene usage by naive B cells and plasmablasts recovered from infected mice on day 10 p.i. Shannon index indicates the diversity of the B cells within each population, and the number of cells within each population is listed above the diagrams. P value represents the median P value of 1,000 runs of Jack knife resampling of χ² tests.