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. 2021 Apr 8;218(6):e20202359. doi: 10.1084/jem.20202359

Figure 4.

Figure 4.

PtS blockade inhibits plasmablast responses and improves humoral immunity. (A–E) C57BL/6 mice were infected with Py and treated with mch1N11 or isotype control from days 5 to 7 p.i. Representative plots and summary graphs showing splenic plasmablasts (CD138hiIgDneg) on day 10 p.i. (A), CD95+GL7+ B (GC) cells (B), and CXCR5+PD1+ CD4 (GC-Tfh) cells on day 21 p.i. (C). n = 4 mice/group; mean ± SD, representative of two biologically independent experiments. (D) Summary graphs showing parasite-specific serum IgG antibody end point titers on day 21 p.i. n = 5 mice/group; mean ± SD, representative of two biologically independent experiments. (E) Summary graph showing frequency of infected RBCs on day 10 p.i. n = 6 mice/group, mean ± SD, pooled from two biologically independent experiments. (F and G) 50:50 bone marrow chimeras using Tim1−/− and WT cells (F) or Axl−/− and WT cells (G) were generated and infected with 1 × 106 Py pRBCs. Representative plots and summary graphs showing frequency of splenic plasmablasts (CD138hiIgDneg) derived from each genotype on day 10 p.i. are shown. Connecting lines match gene knockout and WT B cells within each individual chimeric mouse. Significance was calculated using Mann-Whitney test (A–E) and paired t test (F and G). *, P < 0.05; **, P < 0.01; ***, P < 0.001.