Figure S1.

Clinical correlations and serum neutralization in vaccinees. (A–F) Serum TBEV EDIII ELISA data (IgG) from Fig. 1 B plotted against demographic and available clinical information. (G–L) Serum TBEV RVP neutralization data from Fig. 1 C plotted against demographic and available clinical information. A and G show age (no significance), B and H show length of hospitalization (no significance), C and I show severity of disease (no significant differences), D and J show IgM titers (IP) measured at the time of hospitalization (P = 0.0029 and P < 0.0001, respectively); E and K show IgG titers (Vienna units/ml) measured at the time of hospitalization (P < 0.0001 and no significance, respectively), and F and L show patient gender (no significant differences). Statistical significance was calculated for A, B, D, E, G, H, J, and K using two-tailed Spearman's tests; for L and F using Mann–Whitney tests; and for C and I using one-way ANOVA with Tukey’s test. (M) Correlation between serum TBEV EDIII ELISA (IgG) and RVP neutralization data. (N) TBEV RVP neutralization curves with sera from vaccinated PBMC donors. Representative of two experiments, each performed in triplicate. Data are presented as mean with standard deviation. (O) Summary of serum NT₅₀s for all infected and vaccinated PBMC donors. Related to Fig. 1 and Table S1. RLU, relative light units.