Fig. 2.

Comparative accumulation of β-globin reporter transcripts containing different poly(A) lengths stabilized by the dENE. (A) Schematic of the β-globin reporter constructs containing a cytomegalovirus (CMV) promoter, a human intronless β-globin gene (βΔ1,2), and the dENE, followed by either a bovine growth hormone poly(A) site (BGH pA) (Middle) or a poly(A) sequence of different lengths as indicated on the x-axis of the graph in B and mascRNA (Bottom). The arrowhead indicates the RNase P cleavage site upstream of mascRNA. (B) Northern blot analyses of transcripts expressed in HEK293T cells upon transfection of different β-globin reporter constructs. Blots (Top) were probed for β-globin and neomycin resistance (Neo^R) sequences. Neo^R serves as a transfection and loading control. Quantification of the Northern blots (Bottom) with the β-globin signals normalized to those of Neo^R. Accumulation of βΔ1,2 was set at 1. Accumulations relative to no ENE control are the average of at least three independent experiments ± SD.