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. 2021 Mar 30;118(14):e2026656118. doi: 10.1073/pnas.2026656118

Fig. 5.

Fig. 5.

SAXS experimental data, Kratky plots, and P(r)s of two designed dENE constructs and their complexes: construct B (A) and construct C (B) (see Fig. 1). (C) The aligned comparisons of the SAXS reconstructions of B (lime green) and C (light purple) in the absence of poly(A) and the aligned reconstructions of their corresponding complexes: B+poly(A) (green) and C+poly(A) (gold). Using these reconstructions, we identified the longer stem in construct C and its poly(A)-bound complex and, therefore, established the orientation of both constructs. (Scale bars: 20 Å.) The hairpin of the hairpin poly(A) can be located using the known orientation of B+poly(A) and aligning it with B+hairpin poly(A) (magenta). The first two alignments in D demonstrate that these two complexes have similar lengths while the other alignments, rotated 90 degrees, reveal that the added hairpin extrudes from the complex near the top. These models indicate that the 5ʹ-to-3ʹ directionality of poly(A) is from top to bottom of construct B and that the interaction of the 3ʹ poly(A) terminus is with the lower ENE domain. Cross-sections of each surface model are on the Right.