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. 2021 Mar 30;118(14):e2010333118. doi: 10.1073/pnas.2010333118

Fig. 4.

Fig. 4.

Genetic up-regulation of ICAM-1 in vivo by adenovirus enhances abscopal effect of RT. (A) Schematic illustration of X-RT combined with ICAM-1 Ad. (B) Tumor growth curves of irradiated tumors (IT) and nonirradiated tumors (NIT) in 4T1 tumor-bearing mice after the indicated treatments: control (PBS), ICAM-1 expressing Ad alone (ICAM-1 Ad), RT plus PBS (RT + PBS), RT plus control Ad (RT + control Ad) or RT plus ICAM-1 expressing Ad (RT + ICAM-1 Ad) (n = 6 to 9 per group). (C and D) Flow cytometry analysis assessing ICAM-1+ cells (C) and CD8+/CD4+ cells (D) in the nonirradiated tumors after the indicated treatments (n = 5 per group). (E and F) Flow cytometry analysis evaluating the percentage of IFNγ-secreting CD8+ T cells (E) and the ratio of CD8+/Treg cells (F) in nonirradiated tumors of mice after the indicated treatments (n = 5 per group). All the numerical data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 by two-way ANOVA (B) or an unpaired Student t test (C–F).