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Aspartate ladder regulation of substrate selection is additive. (A) Cartoon schematic of aspartate ladder mutations. (B) Glycosylation of HeLa extracts by D2A-2prox and D2A-3med mutants shows large global increase in glycosylation and preferential increases in different O-GlcNAc bands (see asterisks). These preferences are additive in the D4A mutant. Representative blot for two independent experiments. See methods for activity calculations.
