Fig. 5.

Expression profiles and functional categories of the genes producing high-confidence NAD-RNAs in the different growth phases. (A) Scatterplots showing normalized expression values of NAD-RNAs and non–NAD-RNA transcripts from the same genes in the exponential phase (Left) or stationary phase (Right), with colors of the dots indicating density of the points (blue→red; low→high). (B and C) Overlap between the top 200 most highly expressed genes and the high-confidence NAD-RNA–producing genes in the exponential (B) and stationary (C) phases. (D and E) Bar plots of GO enrichment analysis of the NAD-RNA–producing genes in exponential (B) and stationary (C) phases. The top five terms are shown with their FDR values in −log10 transformation. (F) Comparison of changes (Log2 fold change) in the NAD-RNA levels and the total transcript levels from the high-confidence NAD-RNA–producing genes between exponential and stationary phases. The values in the x and y axes represent Log2 fold differences of NAD-RNA levels and total transcript levels, respectively. The genes whose total RNA levels showed significant differential expression between the two phases are highlighted in red.