Table 1.

Photophysical properties of thienyl-substituted fDAEs in acetonitrile and aqueous (Aq.) buffered solutions (pH = 7, phosphate buffer 100 mM)

	λmaxabs [nm]/ε × 10−3 [M-1cm−1]*		λmaxem λfl,max[nm]	Φfl† CF		Φisom/MeCN		Φisom/Aq. buffer		Φfl/ΦCF→OF
	OF*	CF	CFΦOF→CFc	MeCN	Aq. buffer	OF→CFΦOF→CF‡	CF→OFΦCF→OF§	OF→CFΦOF→CF‡	CF→OFΦCF→OF§	Aq. buffer
fDAE1	360/27.9	487/50.0	578	0.63	0.36	1.0 × 10−2	1.0 × 10−5	2.7 × 10−3	1.0 × 10−5	34,500
fDAE2	360/30.6	491/54.3	575	0.61	0.37	5.3 × 10−3	2.5 × 10−4	2.7 × 10−3	2.9 × 10−4	1,270
fDAE3	369/19.3	504/43.7	608	0.65	0.34	9.2 × 10−4	3.3 × 10−6	2.9 × 10−4	3.8 × 10−6	88,400
fDAE4	368/23.6	506/52.8	605	0.59	0.33	1.1 × 10−3	5.2 × 10−5	3.0 × 10−4	5.3 × 10−5	6,180
fDAE5	370/19.1	503/41.9	606	0.48	0.29	4.6 × 10−4	1.1 × 10−5	4.0 × 10−4	4.3 × 10−6	67,700
fDAE6	372/25.1	505/49.6	606	0.51	0.27	1.2 × 10−3	1.9 × 10−5	3.2 × 10−4	1.6 × 10−5	17,400

^*Lowest energy absorption peak.

^†Fluorescence quantum yield.

^‡Measured at 405 nm or 365 nm.

^§Measured at 505 nm or 530 nm (see SI Appendix for details).