Fig. 5.

The formation of microcalcifications varied significantly with single-dose BiP treatment in a time-dependent manner. Schematic depicting the experimental setup for treating samples with a single dose of BiP at different times (A). The amount of calcium phosphate mineral formed in each sample was measured using a fluorescent-based assay and varied significantly across treatment groups (B, P = 0.0025 via one-way ANOVA; n = 5 biological replicates), with a significant difference between control and day 6 groups (*P = 0.027, error bars represent SD). The chemical composition of microcalcifications formed in each sample was measured using FTIR, with a representative spectrum shown for each group (C).