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. 2021 Mar 31;118(14):e2022756118. doi: 10.1073/pnas.2022756118

Fig. 1.

Fig. 1.

The structure of the Gcn1-bound disome. (A) Schematic representation of the yeast Gcn1 protein with its predicted HEAT repeats (gray boxes). Areas of Gcn1 binding to the ribosome, Gcn20, and Gcn2 are indicated with arrows. (B–D) Cryo-EM reconstruction of the Gcn1-disome complex with segmented densities for Gcn1 (orange) and Gcn20 (yellow). On the leading ribosome, 40S (cyan), 60S (gray), Rbg2 (light blue), Gir2 (green), and Gir2-RWD (cyan); on the colliding ribosome, 40S (pale yellow), 60S (gray), Mbf1 (deep blue), and the Gcn1 interaction with P1/P2-stalk proteins (salmon). (E–G) Molecular models of the Gcn1-bound disome structure (E); a cut-through view of the Gcn1-disome leading ribosome with Rbg2, peptidyl-tRNA (red) in the A-site, deacylated tRNA (green) in the P-site, and eIF5A (purple) in the E-site (F); and the colliding ribosome with Mbf1 (dark blue), peptidyl-tRNA (gold) in the A/P-site, and deacylated tRNA (purple) in the P/E-site (G).