FIGURE 1.

LGOX R305E shows strict specificity for l‐arginine. (a) Blue native‐PAGE analysis of LGOX R305E. In 10 μg of protein was applied. (b) SDS‐PAGE analysis of purified LGOX R305E: lane M, molecular mass marker; lane 1, purified LGOX R305E. (c) Comparison of substrate specificity profiles of LGOX R305E and wild‐type LGOX. Relative oxidative activities of LGOX R305E and wild‐type LGOX are indicated in red, and blue bars, respectively. The substrate that showed the highest activity was set as 100% relative activity. Enzyme activities were measured using 1 mM substrates at 40°C