TABLE 1.

Summary of purification of LGOX R305E

	Total protein (mg)	Total activity (U)	Specific activity (Umg−1)	Yield (%)	Purification (fold)
Crude extract	937	59	0.063	100	1
Protease digestion	125	553	4.4	936	70
Ammonium sulfate (30%–60%)	76	184	2.4	311	38
DEAE Toyopearl 650 M	19	110	5.7	186	91
Sephacryl S 300 HR	13	99	7.5	168	118

Note: The activity of LGOX R305E was measured at 40°C, pH 8.0.