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. 2021 Mar 19;12(10):3067–3076. doi: 10.7150/jca.54759

Figure 3.

Figure 3

Overexpression of miR-369 inhibits HCC cells proliferation. A. The expression of miR-369 in HCC cell lines was checked by real-time PCR assay. B. The overexpression effect of miR-369 in HCCLM3 and Huh7 cells was checked by real-time PCR assay. C. Cell proliferation was measured using CCK-8 assays in HCCLM3 miR-369 mimic or Huh7 miR-369 mimic and their control cells. D. Colony formation assays of HCCLM3 miR-369 mimic or Huh7 miR-369 mimic and their control cells. E. Cell proliferation was assessed using EdU immunofluorescence staining (red) in HCCLM3 miR-369 mimic or Huh7 miR-369 mimic and their control cells. Nuclei were stained with Hoechst 33342 (blue). F. HCCLM3 miR-369 mimic and its control cells were implanted subcutaneously to induce xenograft tumor in nude mice (n=6). Xenografted tumor growth was monitored and tumor weight was measured 6 weeks later. G. Xenograft tumors were excised and subjected to IHC staining.