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. Author manuscript; available in PMC: 2021 Apr 12.
Published in final edited form as: Mol Plant. 2019 Jun 7;12(10):1338–1352. doi: 10.1016/j.molp.2019.05.012

Figure 4. ARR3 contributes to ethylene-induced RAM shortening in an ETR1-dependent fashion.

Figure 4.

Six-day-old seedlings following 24h of hormonal treatment (5 μM BAP, 5 μM ACC, 5 μM BAP + 5 μM ACC and 0.1% DMSO as a control supplemented in liquid media were analyzed. (A, B) Heat maps of each quantified pARR3::YPET-N7 expressing nuclei showing the spatial induction of ARR3 promoter activity in the root of Col-0 (A) and etr1-1 (B) background. The images show results of signal intensities measured in single root per each treatment as a representative example (n=5). Scale bars represent 50 μm. (C) RAM size of Col-0 and the arr3 mutant was determined as a number of cortex cells per file from the quiescent center (QC) to the first elongated cell (mean +/− SE, n=15). The mean numbers of RAM cells are shown at the bottom of the corresponding bar. The statistical significance (t test) of differences between control and hormonal treatments or ACC and BAP treated roots (bracket) at alpha < 0.01 and < 0.001 are denoted by asterisks (** and ***, respectively). ctrl, control; BAP, benzyladenine; ACC, 1-aminocyclopropane-1-carboxylic acid.