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NUCB1 suppressed GEM-induced UPR and autophagy. SW1990 cells overexpressing NUCB1 were treated with 20 μM GEM. (A,B) Western blot was conducted to determine the proteins levels of NUCB1, UPR-associated genes (GRP78, CHOP, p90ATF6, p50ATF6) and autophagy-associated genes (p62, LC3-II/-I, and XBP1). GAPDH was used as a loading control. (C) Immunofluorescence staining was performed to detect LC3 expression. Scale bar: 50 μm. *p < 0.05, ***p < 0.01, ***p < 0.001.
