Figure 6.

METTL3 regulates m⁶A modification of NUCB1 via the m⁶A reader YTHDF2. (A) m⁶A levels in six pairs of PDAC samples and adjacent non-tumorous (N) tissues were measured. ^***p < 0.001 compared with N. (B) Relative m⁶A levels of NUCB1 5′-UTR in SW1990 and CFPAC1 cells were detected by RIP followed by qRT-PCR. ^***p < 0.001 compared with IgG. (C) NUCB1 5′-UTR enrichment in SW1990 and CFPAC1 cells transfected with siRNAs targeting WTAP, METTL3, or METTL14 was determined by RIP followed by qRT-PCR. (D,E) YTHDF2 silencing in SW1990 and CFPAC1 cells increased NUCB1 mRNA levels (D) and stability (E). ^***p < 0.001 compared with siNC. (F) RIP followed by qRT-PCR was performed to examine the interaction between YTHDF2 and 5′-UTR of NUCB1 mRNA. ^***p < 0.001 compared with IgG.