Figure 1.

Characterization of antineuronal antibody staining. Mice were perfused with 4% paraformaldehyde; 12-μm frozen sagittal brain sections were immunostained with cerebrospinal fluid at a 1:4 dilution and counterstained with an antihuman IgG secondary antibody (green) (Jackson #709-545-149 at 2 μg/mL). Nuclei were labeled with DAPI (blue). Scale bars = 10 μm. (A) Cortical immunostaining of pyramidal neuron cell bodies and proximal processes in layer II of the anteromedial cortex. Staining of neuropil was also observed. (Inset) Cerebrospinal fluid immunostains Satb2-expressing (red) neurons (filled arrowheads) but not surrounding Satb2-negative cells (unfilled arrowhead) (Abcam #ab51502 at 1 μg/mL). (B) Relatively uniform punctate staining along the ventricular wall (filled arrowheads) and overlying corpus callosum. (C) Olfactory bulb immunostaining of mitral cell bodies (filled arrowheads) and neuropil of the external plexiform layer (ep). (D) Hippocampal immunostaining of an axon-like process in the hilus (h) of the dentate gyrus (filled arrowheads) and a subset of hilar cell bodies (unfilled arrowheads). (E) Thalamic axon-like (filled arrowhead) and scattered (unfilled arrowhead) punctate immunostaining. (F) Immunostaining of cerebellar Purkinje cell bodies (filled arrowheads) and neuropil of the molecular layer (m). bv, blood vessel; gc, granule cell layer; ip, internal plexiform layer; mc, mitral cell layer; pc, Purkinje cell layer; v, ventricle.