Fig 2. Expression of the LvAPN1 gene upon VPAHPND and VPAHPND toxin challenges.
(A) Stomach, (B) hepatopancreas, and (C) hemocyte of L. vannamei were collected before infection (unchallenged) and after challenge with 5HP (i) or the partially purified VPAHPND toxins (ii) at 12 and 24 h. LvAPN1 expression was analyzed by qRT-PCR using EF-1α as the internal control gene. The data represent fold change of LvAPN1 expression in the 5HP or VPAHPND producing strain challenged group compared to the S02 or non-VPAHPND toxin producing strain-challenge control. The experiments were done in triplicate. Each bar represents the mean ± standard deviation (SD). Asterisks indicate significant difference (P < 0.05).