Fig 6. Binding ability of rPirA^vp and rPirB^vp on immobilized recombinant truncated LvAPN1-His determined by ELISA.

The purified rPirA^vp-His, rPirB^vp-GST or rGST (0–10 μM) was added to a purified recombinant truncated LvAPN1-coated plate, followed by probing with the anti-PirB^vp specific primary antibody and goat anti-rabbit-conjugated HRP secondary antibody. Finally, after addition of the tetramethylbenzidine (TMB) substrate, the absorbance at 450 nm (A₄₅₀) was measured. Solid lines illustrate the fitted curves. The data are shown as the mean ± 1 Standard error of mean (SEM), derived from triplicate experiments.