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. 2021 Mar 31;19(3):e3001169. doi: 10.1371/journal.pbio.3001169

Fig 4. Enhanced H4K8ac in the germline is required for the intergenerational pathogen avoidance.

Fig 4

(A) Whole-mount immunofluorescence using an anti-H4K8ac antibody to strain F1 wild-type N2 young adults from P0 animals exposed to E. coli (E. c), P. aeruginosa (P. a), or heat-killed E. coli (HK E. c) starting at the L4 stage for 24 hours. (B) Lawn occupancy of F1 wild-type N2 animals at 12 hours from P0 animals exposed to E. coli (E. c), P. aeruginosa (P. a), or heat-killed E. coli (HK E. c) starting at the L4 stage for 24 hours (n = 20). Three independent experiments were performed. “n” represents the number of animals for each experiment. “*” asterisk indicates significant difference; *P ≤ 0.05. (C) C. elegans exposure to P. aeruginosa causes an intestinal colonization and distention that leads to H4K8ac in the germline. Both H4K8ac in the germline and the germline itself are required for an intergenerational pathogen avoidance. See S1 Data for the corresponding data. H4K8ac, histone H4 Lys8 acetylation.