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. 2021 Mar 31;17(3):e1009476. doi: 10.1371/journal.ppat.1009476

Fig 4. Intestinal-epithelial LSD1 is required for effective immunity against the whipworm Trichuris muris.

Fig 4

(A) WT (n = 9), cKO (n = 9) and Rag1KO (n = 3) mice were infected with 200 embryonated eggs of T. muris. Worm burden was quantified in caecum after 21 days of infection. Pooled from 2 independent experiments. (B) PAS staining for goblet cells was done in ceacal epithelium of WT and cKO mice after 21 days of T. muris infection. (C) Quantification of PAS+ cells in caecal epithelium of WT (n = 8) and cKO (n = 9) mice after 21 days of T. muris infection. (D) RT-qPCR for Retnlb and Clca1 in proximal colon of WT (n = 8) and cKO (n = 8) after 21 days of T. muris infection. (E&F) Representative confocal images and quantification of RELMβ+ cells (green) in caecum tissue of WT (n = 8) and cKO (n = 9) mice infected with T. muris for 21 days. DAPI (blue) was used as a nuclear counter stain. Scale bar = 50 μm. (G) Measurement of cecal crypts length in WT (n = 8) and cKO (n = 9) mice infected with T. muris for 21 days. (H) Sandwich ELISA for quantification of IL-13 and IFN-γ expression in restimulated mesenteric lymph node cells after 21 days of T. muris infection. Unpaired two-tailed Student’s t test (A, C, D, F&G) and one-way ANOVA (H) was performed to observe significant differences. ns = not significant, * P ≤ 0.05, **, P < 0.01, *** P ≤ 0.001.