Extended Data Fig. 8. Phased cleavages occur on ribosome-bound piRNA precursors.

a, Distance spectrum of 5´-ends of anti-HA immunoprecipitated RPFs from uORFs (left) and from UDRs (right) that overlap 3´-ends of MILI-bound pre-piRNAs in Pnldc1 mutant testis (three biological replicates). Data are mean ± standard deviation. b, Distance spectrum of 5´-ends of anti-HA immunoprecipitated RPFs from uORFs (left) and from UDRs (right) that overlap 3´-ends of MIWI-bound pre-piRNAs in Pnldc1 mutant testis (three biological replicates)⁶. Data are mean ± standard deviation. c, Distance spectrum of 5´-ends of piRNAs from uORFs (left) and from UDRs (right) that overlap 3´-ends of pre-piRNAs in Pnldc1 mutant testis⁶. Experiments have been repeated for two biological replicates and mean is used for plotting. d, Sequence logos depicting the nucleotide bias at 3´-ends and 1 nt downstream of the 3´-ends of total pre-piRNA species (top), MILI-bound pre-piRNA species (middle), and MIWI-bound pre-piRNA species (bottom) from Pnldc1 mutants. Statistical Source Data are provided in Source Data Extended Data Figure 8.