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. Author manuscript; available in PMC: 2021 Apr 12.
Published in final edited form as: J Immunol. 2019 Nov 18;203(12):3237–3246. doi: 10.4049/jimmunol.1900245

Figure 4: Nrp1 expression is induced by oxLDL.

Figure 4:

(A) Splenocytes from ApoE−/− mice were cultured with or without oxLDL or Dil-oxLDL (10 μg/ml) for 3 days. The expression of Nrp1 and CD44, and the uptake of oxLDL were determined using flow cytometry. The top contour plots are gated on live CD4+TCRβ+ cells and show the expression of Nrp1 and CD44. The bottom histograms are gated on Nrp1+CD44+ or Nrp1−CD44− cells and show the uptake of labeled Dil-oxLDL. (B) Bar graphs showing the relative expression of mRNA of Abca1 and Abcg1 in sorted Nrp1+YFP+ or Nrp1−YFP− CD4 T cells. Results are expressed as the mean ± s.e.m from one of two independent experiments (n=6 (A) and n=3 (B)). Statistically significant differences were at * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.