Figure 1.

GBM-CSF effects on SERPINA3 expression and migratory capacity in BTICs. (A) GBM-CSF increases the migratory response of 1A and 965 BTICs compared to noncancer CSF. (B) RNA expression microarray showed that SERPINA3 was one of the genes upregulated on BTICs after conditioning of GBM-CSF. (C) Confirmation of higher levels of SERPINA3 mRNA by RT-qPCR following GBM-CSF stimulus in different BTICs. (D) SERPINA3 KD impairs the migration response of 965 and 1A BTICs to CSF stimulation. *P < .05, **P < .01. BTIC, brain tumor-initiating cell; GBM-CSF, glioblastoma-cerebrospinal fluid; KD, knockdown.