Figure 3.

SERPINA3 effect on proliferation and cancer stem cell characteristics. (A) BTICs with SERPINA3 KD exhibit lower sphere formation capacity than controls (1A and 965 cells). (B) S-phase of cell cycle and (C) colony formation capacity are decreased after SERPINA3 KD in BTICs 965 and 1A. (D) The CD133⁺ population of BTICs has increased expression of SERPINA3 mRNA by RT-qPCR (965, 1A, and 612 cells). (E) SERPINA3 KD decreases CD133⁺, CD44, and CD49f expression in BTICs (965 and 1A cells) (F). *P < 0.05, **P < .01. BTIC, brain tumor-initiating cell; KD, knockdown.