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Global DNA methylation levels in vehicle compared with cadmium-exposed mice. Methylation levels were measured using a 5-methylcytosine -specific antibody ELISA assay on purified DNA from sperm of vehicle and cadmium-exposed mice (n = 6). Data are shown as mean ± SEM (n = 3). A p < .05 determines significance as assessed by a Mann Whitney test. We found exposure to cadmium in drinking water was (nonsignificantly) associated with a 10% reduction of global DNA methylation (p = .09).
