Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 23;180(2):262–276. doi: 10.1093/toxsci/kfab002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

PMC Copyright notice

Figure 5. — Validation by pyrosequencing of specific gene promoter loci of the reduced representation bisulfite sequencing data. A, Cartoon representation of chromosomal and CpG positions for Spata13 (chr14: 60732886) and possible effect of methylation status on transcriptional activity. B, Lollipop representation of bisulfite converted DNA (vehicle vs cadmium, n = 8–10 mice). C, Percent methylation at each of the CpG positions at the Spata13 promoter (top) and the Lefty2 promoter (bottom). D, Cartoon representation of chromosomal and CpG positions for Lefty2 (chr1: 180893911) and possible effect of methylation status on transcriptional activity. E, Lollipop representation of bisulfite converted DNA (vehicle vs cadmium, n = 8–10 mice). Each group (box) depicts a mouse. CpG locations are depicted from left to right and the number of clones after bisulfite pyrosequencing from top to bottom. Erroneous sequences and those with low conversation rate were removed, thus the differences in the sizes of each group (box).