Table 1.

Data Filtering of the CpG Coverage in Sperm of Vehicle Versus Cadmium-Exposed Mice

	CpG Covered in ≥80% of Samples		CpG Covered in All Samples
	Covered >10× (1065 495 CpGs)		Covered >10× (320 000 CpGs)
	Methylation Difference Threshold		Methylation Difference Threshold
q-value	25%	10%	25%	10%
0.01	1788	52 302	69	9429
0.05	1788	52 302	69	9429

We compared different sequence depth (10×) in 80% of the samples versus 100% of the samples. Due to the high variability in animals, CpGs covered in at least 80% of the samples were used for further analysis.