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. 2021 Jan 27;180(2):224–238. doi: 10.1093/toxsci/kfab012

Figure 4.

Figure 4.

Effects of bisphenols on MA-10 mouse tumor Leydig cells. Cells were exposed for 48 h to each chemical followed by fluorescence microscopy using the Operetta High Content imaging system (40× magnification). Images illustrating the effects of: (A) BPA on lysosome numbers, calcein intensity, and cellular oxidative stress in MA-10 cells; (B) BPAF on lysosome numbers in MA-10 cells; (C) BPS on lysosome numbers in MA-10 cells; and (D) BPTMC on lipid droplet numbers in MA-10 cells. Concentrations at which significant changes were seen are displayed. Lysosome numbers, calcein intensity, and cellular oxidative stress were determined using Lysored, Calcein, and Cellrox dye staining, respectively. Lipid droplet numbers were determined using Nilered dye staining (N = 5). Graphs illustrate the results for the effects of BPA on calcein intensity (E), BPA, BPAF, and BPS on lysosome numbers (F), BPA on oxidative stress (G), BPM and BPTMC on lipid droplet area (H), and BPTMC on lipid droplet numbers (I). Values represent the means ± SEM. One-way ANOVA was conducted to determine concentrations where there were significant effects *p < .05, N = 5.