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. 2021 Mar 29;10:e61885. doi: 10.7554/eLife.61885

Figure 1. Summary of the HT-SELEX results in P. aeruginosa.

(A) Schematic description of protein expression and HT-SELEX procedure and output. (B) Network analysis of similarity of the obtained PWMs. Diamonds indicate TF genes, circles indicate individual PWMs. Edges are drawn between a TF and its PWM model, and between similar models if SSTAT similarity score >1.5e-05. To save space, ‘PA’ is omitted in all TFs names. The names of RRs of TCS are marked in pink font. (C) Comparison of the binding motifs of three TFs (LasR, RsaL, and PhoB) obtained from HT-SELEX (upper) and ChIP or cross-species sequence alignment methods (lower). Arrows indicate half-sites in dimeric sites. Also see Figure 1—figure supplement 1, Supplementary file 1.

Figure 1.

Figure 1—figure supplement 1. Ligand design of HT-SELEX and the distribution of the 24 RRs genomic binding, related to Figure 1A and Figure 1B, and Figure 2D.

Figure 1—figure supplement 1.

(A–D) The schematic diagram for generating the input library for HT-SELEX. (E) The location and the number of binding sites for 24 RRs. The horizontal axis represents the name of each RR, and the positions annotation of 24 different RRs binding sites in the P. aeruginosa genome using pie charts, and the area of each pie chart represents the number of binding sites (log2-transformation).