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. 2021 Apr 8;58(6):28. doi: 10.3892/ijo.2021.5208

Figure 4.

Figure 4

MET reverts mTOR activation induced by cisplatin in A549 lung cancer cells. (A) Analysis of protein content of the mTOR signaling pathway in A549 lung cancer cells after treatment with 10 µM CIS for 72 h and 10 mM MET for 72 h analyzed via western blotting. Band densitometry performed using ImageJ of the protein expression levels of (B) p-mTOR/mTOR (n=2), (C) p-AMPK/AMPK (n=2), (D) p-S6K1/S6K1 (n=3) and (E) p-S6/S6 (n=3). *P<0.05 vs. C; #P<0.05 vs. CIS. CIS, cisplatin; C, control; MET, metformin; p, phosphorylated; a.u., arbitrary units.