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. 2021 Apr 5;2021:6665601. doi: 10.1155/2021/6665601

Table 1.

The forward and reverse primer sequences of various mRNA genes.

Gene Primer sequence Amplicon size (bp)
β-Actin (housekeeping gene) F: 5′-TCACCCTGAAGTACCCCATGGAG-3′ 151
R: 5′-TTGGCCTTGGGGTTCAGGGGG-3′
TNF-α F: 5′-AAAATCCTGCCCTGTCACAC-3′ 323
R: 5′-GCTGAGGTTGGACGGATAAA-3′
iNOS F: 5′-ATGGAACAGTATAAGGCAAACACC-3′ 220
R: 5-′GTTTCTGGTCGATGTCATGAGCAAAGG-3′
MMP-9 F: 5′-CTGGGCTTGATGCCTGTTT-3′ 331
R: 5′-TTGTGGTGGTGCCACTTGA-3′