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. 2021 Apr 2;2021:6653387. doi: 10.1155/2021/6653387

Figure 4.

Figure 4

USP22 served as the functional protein of circFAT1(e2)/miR-30e-5p. (a) The relative fluorescence intensity was confirmed by the luciferase reporter assay when the cotransfection between USP22 3′-UTR wild-type or mutant and miR-30e-5p or control happened. (b) There was difference between the expression of USP22 in normal tissues and that in NSCLC tissues. (c) After overexpression of miR-30e-5p, USP22 mRNA levels decreased. (d) USP22 mRNA level decreased after knockdown of circFAT1(e2) in A549 and H1299 cells. (e) USP22 mRNA expression can be increased by the miR-30e-5p inhibitor which can also resist the reduction of USP22 mRNA caused by circFAT1(e2) knockdown. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.