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Morphology of the cells derived from PANC-1. A, PANC-1 cells cultured in DMEM/F-12 show adherent morphology. B, PANC-1 cells were suspended in the sphere induction medium and incubated for a week as described in Materials and Methods. C, Obtained PANC-1 spheroids were transferred to the laminin-coated dishes and incubated with CSC culture medium for a month.
