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ERK1/2 phosphorylation levels in P-CSLCs. Representative p-ERK1/2 (44/42 kd) protein bands tested using Western blot assay in PANC-1 and PANC-1-Lm (A). Valosin-containing protein (VCP) was used as a loading control. B, Band densities were quantified followed by normalizing to VCP as described in Materials and Methods. The intensities are represented as relative values to PANC-1.
