Table 1.
Detection of respiratory viruses using the AdvanSure RV plus real-time PCR and Real-Q RV II Detection assays
| Virus | Percent agreement* | Kappa value | ||||
|---|---|---|---|---|---|---|
| Positive | Negative | Observed kappa value | 95% CI | |||
| % (N) | 95% CI | % (N) | 95% CI | |||
| ADV | 100 (24/24) | 82.8–100 | 100 (312/312) | 98.5–100 | 1.00 | |
| INF A | 100 (24/24) | 82.8–100 | 99.7 (311/312) | 97.9–100 | 0.98 | 0.94–1.00 |
| INF B | 100 (12/12) | 69.9–100 | 100 (324/324) | 98.5–100 | 1.00 | |
| PIV 1 | 100 (15/15) | 74.7–100 | 100 (321/321) | 98.5–100 | 1.00 | |
| PIV 2 | 100 (15/15) | 74.7–100 | 100 (321/321) | 98.5–100 | 1.00 | |
| PIV 3 | 100 (18/18) | 78.1–100 | 97.5 (310/318) | 94.9–98.8 | 0.81 | 0.68–0.94 |
| HRV | 84.4 (38/45) | 69.9–93.0 | 99 (288/291) | 96.8–99.7 | 0.87 | 0.79–0.95 |
| RSV A | 83.3 (15/18) | 57.7–95.6 | 100 (318/318) | 98.5–100 | 0.90 | 0.80–1.00 |
| RSV B | 100 (23/23) | 82.2–100 | 100 (313/313) | 98.5–100 | 1.00 | |
| HMPV | 92.9 (26/28) | 75.0–98.8 | 99.7 (307/308) | 97.9–100 | 0.94 | 0.87–1.00 |
| CoV 229E/OC43 | 100 (28/28) | 85.0–100 | 98.4 (303/308) | 96.0–99.4 | 0.91 | 0.83–0.99 |
| CoV NL63 | 100 (13/13) | 71.7–100 | 99.4 (321/323) | 97.5–99.9 | 0.93 | 0.82–1.00 |
| BoV | 93.8 (15/16) | 67.7–99.7 | 99.4 (318/320) | 97.5–99.9 | 0.90 | 0.80–1.00 |
| ETV | 50 (3/6) | 13.9–86.1 | 98.5 (325/330) | 96.3–99.4 | 0.42 | 0.09–0.75 |
*The Real-Q RV II assay was used as the comparative assay to calculate the positive percent agreement and negative percent agreement.
Abbreviations: ADV, adenovirus; INF A, influenza A virus; INF B, influenza B virus; PIV 1- 3, parainfluenza virus types 1 to 3; HRV, human rhinovirus; RSV A, respiratory syncytial virus A; RSV B, respiratory syncytial virus B; HMPV, human metapneumovirus; CoV, coronavirus; BoV, bocavirus; ETV, enterovirus.