Fig. 3.

TLC of AaTAT assays using various putative substrates which turned out to be accepted by AaTAT. The five amino donors l-Tyr, l-Phe, l-DOPA, l-Trp and l-Ala (each with 2-oxoglutarate as amino acceptor substrate) as well as the amino acceptor prephenate with l-glutamate as amino donor substrate were incubated for 0, 5, 15 and 45 min. Glutamate is not detectable in 0 min assays. With increasing incubation time the substrate decreases and glutamate increases. Turnover rates can be estimated from the color differences. In contrast, the prephenate assays, as they are structured differently, show an increasing arogenate spot in the case of l-glutamate excess. The TLC plates were stained by spraying with 0.3% ninhydrin solution