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. 2021 Apr 12;4:478. doi: 10.1038/s42003-021-01944-2

Fig. 3. The reported LBR N-term mutation affects the Xi localization but not gene silencing in random XCI.

Fig. 3

a Left, Xi-lamina localization is shown by means of H3K27me3/LaminB1 IF. H3K27me3, green; LaminB1, red; DAPI, blue. Dashed boxes indicate Zoom-in images in the top right corner. Arrows indicates the inactive X chromosome. Scale bar indicates ~25 µm. b Xi-centre-LaminB1 distance quantification (n = 681). Samples are color-coded as shown in the legend. Error bars represent standard deviation of the mean (SEM). c qRT-PCR of XCI-silenced and escapee genes26, 27. Analyzed genes are shown. Data from three biological samples are shown. * indicates statistical significance using a two-tailed t-test (p ≤ 0.05). Error bars represent standard deviation of the mean (SEM). p-values: MeCp2, p = 0.039; Dlg3, p = 0.020, Nkap, p = 0.011. Gapdh has been used as an internal normalization control.