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. 2021 Apr 12;12(4):389. doi: 10.1038/s41419-021-03673-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Pearson correlation between the expression of IQGAP2 and IQGAP1 in tissues of breast cancer (r = −0.56, n = 38). B Pearson correlation between the expression of IQGAP2 and phospho-ERK (r = −0.63, n = 10) in tissues of breast cancer. C Pearson correlation between the expression of IQGAP1 and phospho-ERK (r = 0.49, n = 10) in tissues of breast cancer. D Pearson correlation between the expression of the ratio of IQGAP2/IQGAP1 and phospho-ERK (r = −0.64, n = 10) in tissues of breast cancer. In A–D solid and dashed line show regression line and standard error, respectively. E Representative IHC images of IQGAP2, phospho-ERK, and IQGAP1 in the normal and tumor region of a breast cancer patient. F Western blot images showing status of IQGAP2 in pull-down assay performed with IQGAP1_GST and GST only in MCF7 cells. The blots were probed with anti-IQGAP1, anti-IQGAP2, and GST antibodies. G Western blot images showing status of IQGAP2 in pull-down assay performed with IQGAP1_GST and GST only in Hela cells. The blots were probed with anti-IQGAP1, anti-IQGAP2, and GST antibodies. H Left panel shows Western blot of phospho-ERK in MCF7 cell having IQGAP2 overexpression (IQGAP2_Ex), IQGAP2 empty vector (Control_EV), IQGAP2 overexpression with IQGAP1 empty vector (IQGAP2_Ex + G1_EV), overexpression of IQGAP2 on IQGAP1 overexpression (IQGAP2_Ex + IQGAP1_Ex) background, and IQGAP1 overexpression (IQGAP1_Ex). Right panel shows corresponding densitometry data (n = 3). I Western blot of phospho-ERK in MCF7 cell having depletion of IQGAP1 and IQGAP2 (IQGAP2_KD + IQGAP1_KD), depletion of IQGAP2 with control vector for IQGAP1_KD (IQGAP2_KD + Control_Sc), IQGAP2 depletion (IQGAP2_KD), and its control vector (Control_Sc). J Western blot (left) showing expression of IQGAP1 in MCF7 with IQGAP2 reduction (IQGAP2_KD) and its control (Control_Sc). The bar graph (right panel) shows corresponding densitometry data (n = 3). K Western blot (left) image for IQGAP1 expression in MCF7 with IQGAP2 overexpression (IQGAP2_Ex) and its control (Control_EV). The bar graph (right panel) shows corresponding densitometry data (n = 3). Data are presented as mean ± SEM. Student’s t test, two-tailed unpaired was used for the comparison of means. ^#p > 0.05, *p ≤ 0.05.