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. 2021 Apr 12;11:7970. doi: 10.1038/s41598-021-87021-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of expression of selected genes in hBMECs. Comparison was performed between hBMECs induced by S. pneumoniae wild type (SP WT) and S. pneumoniae Δ Lbp (SPΔLbp) using qRT-PCR. Comparison was also performed between hBMECs induced with GFP and Lbp using same set of the genes. Statistically significant difference (p < 0.01, two-tailed p value) was calculated by unpaired t test. Statistics was performed with on-line statistics tool of GraphPad https://www.graphpad.com/quickcalcs/ttest1.