Skip to main content
. 2021 Apr 12;11:7970. doi: 10.1038/s41598-021-87021-4

Table 1.

Primers used in the study.

No Protein/(Gene) Primer sequence (5′–3′) Primer efficiency Amplicon length (bp) Annealing temperature (°C)
Primers designed to perform qRT-PCR for gene validation
1 C–C motif chemokine ligand 2 (CCL2) CCL2- sense CAATCAATGCCCCAGTCACCT 94.99% 175 55
CCL2- antisense TCCTGAACCCACTTCTGCTTG
2 Vascular cell adhesion molecule 1 (VCAM1) VCAM1- sense CCCTGAGCCCTGTGAGTTTT 98.01% 138 60
VCAM1- antisense GGCCACCACTCATCTCGATT
3 Interleukin 8, C-X-C motif chemokine ligand 8 (CXCL8) CXCL8- sense CTCCAAACCTTTCCACCCCA 94.99% 153 55
CXCL8- antisense TTCTCCACAACCCTCTGCAC
4 Serum amyloid A2 (SAA2) SAA2- sense CCTTGGTCCTGAGTGTCAGC 96.66% 150 60
SAA2- antisense CATAGTTCCCCCGAGCATGG
5 Serum amyloid A1 (SAA1) SAA1- sense TTTTGATGGGGCTCGGGAC 95.02% 150 60
SAA1- antisense TCGGTGATCACTTCTGCAGC
6 Interleukin 1 receptor like 1 (IL1RL1) IL1RL1- sense AAGGTACAGGGCGCACAAG 93.29% 150 60
IL1RL1- antisense CCTTGCTCATCCTTGACCGT
7 Cyclin-dependent kinase 6 CDK6- sense CCGTGGATCTCTGGAGTGTTG 98.10% 117 60
CDK6- antisense GGAGTCCAATCACGTCCAAGA
8 Collagen alpha-1 COL12A1- sense GACCAGCACTTCCCTCAAAGA 95.22% 114 60
COL12A1- antisense GGTCGGGTAGTTTCTTGAGCA
9 Dehydrogenase/reductase SDR family member 2 DHRS2- sense GCTGTCATCCTGGTCTCTTCC 96.54% 113 60
DHRS2- antisense AGCTCCAATGCCAGTGTTCTA
10 Calponin-1 CNN1- sense TGCGAATTCATCAATAAGCTGCA 97.12% 112 60
CNN1- antisense ACTTGGTGATGGCCTTGATGA
11 Carboxypeptidase A4 CPA4- sense ATTATGGAGGAAGACGCGGTC 97.67% 123 60
CPA4- antisense TACACTTCGGAGCAAGGGTTG
12 β-microglobulin (house-keeping gene) (b2m) b2m- sense GCTCGCGCTACTCTCTCTTT 98.84% 134 55
b2m- antisense CGGATGGATGAAACCCAGACA
Primers designed to knock-out Lbp gene (SPΔLbp)
13 F1 fragment (351 bp upstream to Lbp gene) Ko Lbp-F1 F CCTTAGCCTACTTGATCCTGCT - 351 60
Ko Lbp-F1 R TTCTACTCCTGAAAATTTAATCTGTCAAG
14 Bla gene Ko Bla F CTTGACAGATTAAATTTTCAGGAGTAGAAATGAGTATTCAACATTTCCGTGTCG - 913 60
Ko Bla R TGTCATTTTGTGGGTCTGACTCTTTACCAATGCTTAATCAGTGAGGC
15 F2 fragment (300 bl downstream to Lbp) Ko Lbp-F2 F AGAGTCAGACCCACAAAATGACA - 300 60
Ko Lbp-F2 R CAATTTGTTCGGCGTTGATCC