Fig. 3. Effects of NEIL1 single mutations on RNA structure.

a Editing site is indicated by blue circle and the mutation site is marked by red circle. The editing level shown is the average value from six biological replicates. The single mutations were grouped into six types: sequence change (transition or transversion) without change in the structure, the sequence change (transition or transversion) resulted in breaking of the base pair at the mutation site (break), or resulted in breaking more than one base pair or forming of new base pair(s) (shift). E editing site, M mutation site. b Position-specific effects of NEIL1 single mutations, categorized by six types: transition mutation that does not affect RNA secondary structure (transition, blue square), transition mutation that disrupt the base pair at the mutation site (transition + break, blue triangle), transition mutation that leads to disruption of more than one base pair and/or formation of new base pair (transition + shift, blue cross), transversion mutation that does not affect RNA secondary structure (transversion, red square), transversion mutation that disrupt the base pair at the mutation site (transversion + break, red triangle), transversion mutation that leads to disruption of more than one base pair and/or formation of new base pair (transversion + shift, red cross). The Z-score is calculated for the NEIL1 RNA library as described in “Methods” and the WT editing level Z-score is 0.