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Primers used for real-time RT-PCR
| Name | Direction | Sequence (5′–3′) |
|---|---|---|
| ALP | Forward | TGGACGGTGAACGGGAAAAT |
| Reverse | TAGTTCTGCTCATGGACGCC | |
| RUNX2 | Forward | CCCAGTATGAGAGTAGGTGTCC |
| Reverse | GGGTAAGACTGGTCATAGGACC | |
| COL1A1 | Forward | TGGCAAAGACGGACTCAAC |
| Reverse | GGCAGGAAGCTGAAGTCATAA | |
| GAPDH | Forward | GCACCGTCAAGGCTGAGAAC |
| Reverse | TGGTGAAGACGCCAGTGGA |
